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1

流式細胞分析儀 Flow analyzer

(需線上預約)

BD, FACSCantoII

以液流聚焦方式使細胞(粒子)在液柱中排隊通過雷射光束，產生的光訊號再經由電子系統轉換得各細胞特性參數(包含粒子大小、顆粒性及相對螢光的強度)。

A benchtop cell analyzer, equipped with 2 lasers.
1. Laser excitation wavelength: 488, 633nm.
2. Fluorescence detectors: 6 PMTs.
3. Flow rate: 10, 60, 120ul/min.
4. Sample capability: 5mL BD Falcon Polystyrene round-bottom tube.
5. Autosampler: 40-tube carousel.

Quick guide

Spec. sheet

On-line reservation system

2

聲波聚焦流式細胞分析儀 
Flow analyzer

Thermo Fisher Scientific ,

較傳統流式細胞分析儀之流體聚焦，多增加了聲波輔助聚焦，使高流速時訊號變異度仍低。

A benchtop cell analyzer, equipped with 4 lasers and acoustic-assisted hydrodynamic focusing apparatus.
1. Laser excitation wavelength: 405, 488, 561, 637nm
2. Fluorescence detectors: 14 PMTs.
3. Flow rate: 12.5, 25, 100, 200, 500, 1000ul/min.
4. Sample capability: 1.5-2mL eppendorf, micro tube, 5mL tube (ex. BD Falcon Polystyrene round-bottom tube)

Quick guide

Spec. sheet

Fluorophore Selection Guilde

On-line reservation system

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溫度梯度核酸增殖儀
TGradient PCR

聚合酵素鏈鎖反應，可以溫度梯度測試理想反應溫度(annealing temp.)

1. Through a temperature gradient setting, it allows the detection of the perfect annealing temperature in a single PCR run.
2. Temp. gradient: 40 °C.
3. Temp. range: -3 – 99.9°C.
4. Sample capacity: 0.2ml PCR tubes (max. 96), 96-well microplates or strips.

Biometra_TGradient_user manual

核酸複製同步定量序列偵測系統
Real-time PCR(需線上預約)

在PCR過程中，以螢光即時偵測核酸量，從而將目標基因絕對或相對量化，同時還可通過分析溶解曲線對擴增的核酸進行後PCR分析。96/384 well樣品處理

1. Perform gene quantification (relative or absolute), genetic variations analysis or other applications in need of detecting FL intensity in each PCR cycle.
2. Excitation source: Xenon lamp (430-630nm).
3. Excitation filters: 440, 465, 498, 533, 618nm.
4. Emission filters: 488, 510, 580, 610, 640, 660nm.
5. Detection: CCD camera.
6. Max. heating rate: 4.4 °C/sec.
7. Max. cooling rate: 2.2 °C/sec.
8. Sample capacity: 96- or 384-well PCR plate.

LightCycler480 II_Manual_V1.5

HRM_strategy

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奈米分光光度計
Nanodrop 1000

PerkinElmer
ND-1000 Spectrophotometer

微量(1.5μl)全光譜測定. 可接受濃度2ng~3700 ng/µL 

1. Quantify DNA, RNA and protein samples in seconds with 1-2µL.
2. Light source: Xenon lamp (220-750nm).
3. Sample Vol. range: 1-2µL.
4. Sample no. per measurement: 1.
5. Detection dynamic range: 2-3700 ng/µL for dsDNA.
6. Abs. range: 0.02-75 Abs.

Notice before usage

ND 1000 Manual v3.7

Cuvette及微量(0.5-2μl)全光譜測定, 一次1個樣本, 可接受濃度2-15000 ng/μl dsDNA

1. Quantify DNA, RNA and protein samples in seconds with 1-2µL.
2. Light source: Xenon lamp (190-840nm).
3. Sample Vol. range: 1-2µL.
4. Sample no. per measurement: 1.
5. Detection dynamic range: Pedestal: 2-15,000 ng/µL for dsDNA, Cuvette: 0.4-750 ng/µL for dsDNA.
6. Abs. range: Pedestal: 0-300 Abs, Cuvette: 0-1.5 Abs.

3

奈米分光光度計
Nanodrop 8000

Thermo Scientific, Nanodrop 8000 Spectrophotometer

微量(1-2μl)全光譜測定, 一次1-8個樣本, 可接受濃度2.5-3700 ng/μl dsDNA

1. Quantify DNA, RNA and protein samples in seconds with 1-2µL.
2. Light source: Xenon lamp (220-750nm).
3. Sample Vol. range: 1-2µL.
4. Sample no. per measurement: up to 8.
5. Detection dynamic range: 2.5-3700 ng/µL for dsDNA.
6. Abs. range: 0–75 Abs.

ND 8000 Manual

4

BioRad,
Model 680

96孔微量盤濾片組吸光值(濾片組)讀取

1. Detection mode: Absorbance (Abs).
2. Sample capacity: 96-well microplate.
3. Light source: Tungsten halogen lamp.
4. Detector: Silicon photodiodes.
5. Wavelength selection by filters: 415, 450, 490, 595, 655nm.

Model 680_quick guide in Chinese

多功能微量盤分析儀Microplate reader
(multi-detection)

96孔微量盤全光譜吸光值暨螢光、冷光三種讀取模式

1. Detection mode: UV-Visible Absorbance (Abs), Fluorescence Intensity (FI), Luminescence (Lum), Time-Resolved Fluorescence (TRF), Fluorescence Polarization (FP).
2. Sample capacity: 6-384 well microplate or cuvette.
3. Light source: Xenon lamp.
4. Detector: PMTs.
5. Wavelength selection by monochromators.
6. Abs. wavelength: 200-1000nm.
7. FI wavelength: 250-850nm.
8. Lum wavelength: 250-850nm.
9. FP wavelength: 300-750nm.

M5 manual in Chinese

M5 software User guide

M5 specification

自動分注多功能微量盤分析儀: 含各種偵測功能，具有螢光、高感度的冷光、吸收光、TRF、AlphaScreen/AlphaLISA偵測功能及溫控裝置。；可做全光譜的掃描。

1. Detection mode: UV-Visible Absorbance (Abs), Fluorescence Intensity (FI), Luminescence (Lum), Time-Resolved Fluorescence (TRF), AlphaScreen.
2. Sample capacity: 6-384 well microplate.
3. Light source: Xenon lamp.
4. Detector: PMTs.
5. Wavelength selection by monochromators.
6. Abs. wavelength: 230-1000nm.
7. FI wavelength: 230-850nm.
8. Lum wavelength: 250-850nm.
9.Injector no./Vol. range: 2 / 1 - 475 μl per stroke.

Dispenser quick guide

EnSpire_quick guide in Chinese

EnSpire(2300)_manual

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高效液態層析儀

Waters

四相溶媒混合液態層析儀，配備UV、螢光偵測器、自動進樣系統與強大分析軟體以分析生物分子

超高效液態 層析儀

Waters ACQUITY UPLC  H_Class

具有四相溶媒混合的靈活性和簡易性，並設計直接進樣的注射針的取樣器，可提供UPLC先進的分離性能——高解析度、靈敏度和高通量的先進系統

The sophisticated ACQUITY UPLC H-Class System, in combination with sub-2-μm packing technology, provides the highest resolution of any quaternary LC System. It also improves the characterization of most complex samples through enhanced resolution. For example, more than 20 AccQTag derivatized amino acids can be fully identified with 1 microliter volume injection at 10 pmol range detection within 10-min run. 
Features:

1. Flexibility of multi-solvent blending with the Quaternary Solvent Manager (QSM)
2. High-precision injections with direct inject sampling
3. Accurate temperature control across the entire column
4. Low-dispersion detection capabilities
5. Gradient SmartStart
6. Supported in Empower 3

Link

 User Guide

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1

電泳照膠系統
Geneflash (for  EtBr stain)

Syngene, Geneflash

UV(302 nm)激發, orange filter (605±25 nm)接收, 適合EtBr stain照膠影像擷取

1. For (documenting or digitalizing or detection) EtBr stain gel samples
2. Excitation: 302nm
3. Emission Filter: 605±25nm

2

核酸電泳照膠系統
BioDoc-It Imaging System (for  non-EtBr stain)

UVP, BioDoc-It Imaging System

UV(302, 365 nm)激發, green filter (535±25 nm)接收, 適合非EtBr stain照膠影像擷取。另備有orange filter(605±25 nm)

1. For (documenting or digitalizing or detection) non-EtBr stain gel samples
2. Excitation: 302, 365nm
3. Emission Filter: 535±25, 605±25nm

Quick guide

3

冷光螢光影像分析系統
BioSpectrum 600 Imaging System

UVP, BioSpectrum 600

高階螢光冷光照膠影像擷取分析

1. For (documenting or digitalizing or detection) gel or membrane of chemiluminescence, fluorescence and colorimetric.
2. CCD camera/lens: monochrome, 16-bit, 3.2Mpixels/F1.2, 50mm.
3. Light source: Epi- 365, 488nm, white; Transilluminator- 302nm, white.
4. Emission Filter: Set 1- 485-655, 515-570, 570-640nm; Set 2- 513-557, 565-625, 607-682nm.

Technical Spec.

4

螢冷光影像擷取系統
ImageQuant LAS4000 biomolecular imager

GE, ImageQuant LAS4000

1. For (documenting or digitalizing or detection) gel or membrane of chemiluminescence, fluorescence and colorimetric.
2. CCD camera/lens: monochrome, 16-bit, 3.2Mpixels/F0.85 43mm.
3. 3. Light source: LED Epi-blue, green, red, white; Transilluminator-302nm.
4. Emission Filter: Y515, 575DF20, R670, 605DF40nm.

User Manual

Brochure with specification

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Regarding Usage of Seahorse XFe24@ICOB

User manual in Chinese

Assay kits introduction

Consumables Ordering information

Seahorse XFe24 learning center

On-line reservation system

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生化分析儀

Agilent 2100 Bioanalyzer

儀器由一組卡匣，電壓或壓力控制系統，雙光束的雷射光檢測系統及控制儀器的電腦與容易使用的軟體及標準化的晶片及試劑組合而成。使用者可依不同的樣品種類，如RNA, Cy5 labeled nucleic acids ,and proteins選擇不同的微流體晶片來分析樣品。

User Manual

RNA Quality Control Request Form

DNA Sizing and Analysis Request Form

Protein analysis Request Form

Thermo Savant

Speed Vac

微流體電泳分析儀

LabChip GX是利用微流體晶片所發展出來的快速核酸分析儀。可以在短時間內分析核酸的濃度及純度。取代步驟複雜、耗費人力、時間及無法準確定量的傳統膠片電泳。

 Link

on-line reservation system

4

XcelVap為全自動的吹氮濃縮加熱裝置，結合加熱與自動氮氣壓力控制技術，並主動將蒸氣排出，可快速並溫和地濃縮樣品，最多可同時濃縮54個樣品，濃縮體積最高可達200毫升，大幅提高實驗室產能。可用於液液萃取法、連續式萃取法、固相萃取法或固相微萃取法等任何需要快速濃縮萃取液的實驗。

The XcelVap® is an automated evaporation and concentration System.
Features:

1. Automated pressure profiling for optimum speed with no splattering or cross contamination.
2. Nozzles can be individually changed or reconfigured for different racks by the user.
3. Nitrogen blow-down evaporation of up to 54 sample extracts, ranging in size from less than 1 mL up to 200 mL

FastPrep-24 5G is a versatile sample disruptor designed to quickly and efficiently homogenize, grind, or lyse biological samples by shaking the samples in a tridimensional motion.

1. Shaking speed: 4 m/s - 10 m/s.
2. Shaking mode: tridimensional.
3. Time of per cycle: 1 - 120 s.
4. Cycle no.: 1 - 9 cycles.
5. Sample holder: 24x 2ml QuickPrep Adapter, 24x 2ml CoolPrep Adapter.

Quick guide in Chinese

Instruction manual in English

Suggested parameter settings